FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_Neg.8.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_Neg.8.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3796
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT581.5279241306638567No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG70.18440463645943098No Hit
ACGAATCCCCGACCCTTGGCCAGCTGCTTGTTGGCATCGGTAACCGATGG40.10537407797681769No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC40.10537407797681769No Hit
ATCCAGTAGATGAGATATATCGCGATTATTACAACAATATATTCAAAGAA40.10537407797681769No Hit
CACGAGGACACACCGTCCGTCCACGGCCTTGAGATCGTTCCACGCATGCG40.10537407797681769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAGA100.0068135723145.02
GATCGTC100.0068135723145.07
GTGAGAA100.0068135723145.03
TTCAGTC100.0068135723145.05
Produced by FastQC (version 0.11.5)