FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_Neg.7.20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_Neg.7.20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3603
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT431.1934499028587289No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC80.22203719122953097No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG60.16652789342214822No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT60.16652789342214822No Hit
TATTATTACTTTTTCCTGAAAATCATAACATATATGAGTATATAAAAAAT40.11101859561476549No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC40.11101859561476549No Hit
TAACAATACAATTGCCACAATTTGCCATTGATAACGATTTATGGGTAAAA40.11101859561476549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT200.005919446229.035-39
CCGTCTT200.005919446229.045-49
GAGCCCA200.005919446229.015-19
CACGAGA200.005919446229.020-24
ACGAGAC200.005919446229.020-24
TGCTTGA200.005919446229.055-59
CGTATGC200.005919446229.040-44
GCATGAT200.005919446229.030-34
GCTTGAA200.005919446229.055-59
TCGTATG200.005919446229.040-44
ACTAGGC200.005919446229.025-29
TCTCCGA200.005919446229.010-14
AGACTAG200.005919446229.025-29
TCTTCTG200.005919446229.050-54
AAAAAAG200.005919446229.065-69
CTTCTGC200.005919446229.050-54
CTCCGAG200.005919446229.010-14
GACTAGG200.005919446229.025-29
AAAAAAA1306.05749E-410.03846260-64
Produced by FastQC (version 0.11.5)