FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_Neg.3.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_Neg.3.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3580
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT571.5921787709497208No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC120.33519553072625696No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC110.3072625698324022No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT90.25139664804469275No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG90.25139664804469275No Hit
CCACCCGGGCATGGCCTTCCGGCGTGGCAACCGACGAACCGCGCAACACG40.11173184357541899No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATATCGTATGCCGT40.11173184357541899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC203.553167E-4108.753
CATCTCC203.553167E-4108.759
ACATCTC203.553167E-4108.758
TTATACA203.553167E-4108.752
TACACAT203.553167E-4108.755
ACACATC203.553167E-4108.756
CTTATAC203.553167E-4108.751
CACATCT258.629705E-487.07
ATACACA258.629705E-487.04
AAAAAAA1707.144309E-48.52941160-64
Produced by FastQC (version 0.11.5)