FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_Neg.2.28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_Neg.2.28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6056
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT500.8256274768824308No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG140.2311756935270806No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC130.21466314398943198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC100.006749006146.200011
CATAGCG100.0069199996144.991742
AAAAAAA2550.0054989986.2545457115-119
Produced by FastQC (version 0.11.5)