FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_9361.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_9361.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29922254
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG142900.016.844687145
TATACTG303750.016.1361735
CTCCGAC72950.015.902145
TACACTG222700.015.7252325
CTACACT174150.014.3636334
GTGTAAG166500.013.719341
TCTCCGC161100.013.6365385145
CTATACT197750.013.52944
TCTATAC200900.013.3894473
GTGTAGG129100.013.3686521
GTATAGG107850.013.3131851
CTAGGAC117050.013.0701263
GTATAGA198700.012.992391
TAGACAG352000.012.4618595
TACACAG270750.012.4256665
GTATTAG208700.012.4045971
CCTACAC130350.012.4040213
TAATACT330300.012.402484
GTCCTAG113700.012.373091
TCTACAC183200.012.3480333
Produced by FastQC (version 0.11.5)