FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_7920.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_7920.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24426167
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG312100.016.4480515
TCTCCGC71450.016.134834145
ATGCCGG57050.016.013435145
CTATACT192350.014.6632324
TACACTG224900.014.636645
CTACACT163550.014.4523794
TACACAG269550.013.6915755
ATAGGCG34300.013.5288495
CTAGGAC114700.013.2747253
TCTATAC179850.013.2634073
CCTATAC97900.013.1827773
GTATAGA181550.013.1416591
GTATAGG101550.013.1397911
ATACTGT395800.013.1346996
CCTACAC110200.013.0930593
GTATAAG166300.012.9949481
TAACACG58150.012.9674914
CTAATAC188950.012.9316113
CTAGACT141000.012.5985124
ATACCGT33000.012.5238456
Produced by FastQC (version 0.11.5)