FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_7651.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_7651.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29605578
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG296450.015.1394125
TACACTG206950.015.0300935
TACACCG79900.014.8821915
CTATACT223550.014.7897974
TATACCG60100.014.115015
CTACACT172150.013.5619114
GTATAGA227250.012.9876771
GTATTAG228200.012.8382761
TAGACAG286650.012.6217165
TCTATAC225650.012.5957123
TATACAG274350.012.5533165
CTTACAC166700.012.5264753
CTAGGAC101000.012.4910763
GTACTAG85300.012.4120861
GTGTAGG136600.012.3162441
CCTACAC140700.012.31617453
ATGCCGG155500.012.309084145
TAGACTG159800.012.2959575
CTAGTAC91500.012.2031413
GTATAGG143450.012.1830911
Produced by FastQC (version 0.11.5)