FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_7431.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_7431.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26076730
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT193100.015.0190614
TATACTG255850.014.9061745
TACACTG173900.014.8428165
CCTATAC118600.013.5104333
CTACACT148750.013.3554544
GTGTAGG127300.013.1010261
TACACCG84900.012.9808095
TAGACAG250850.012.8910015
GTATAGA196850.012.7820331
GTATAGG119200.012.7746471
TATACAG237200.012.562995
CTAGGAC84950.012.5462993
TATACCG63150.012.514665
TAGCGCG69150.012.162328145
GTGTAGA167850.011.8800221
GTATAAG176300.011.84531
TCTACAC168550.011.829483
TACACAG210100.011.7677725
TAATACT309850.011.7233374
CTAATAC206050.011.7174563
Produced by FastQC (version 0.11.5)