FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_7379.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_7379.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36938266
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG190600.015.748486145
TATACTG361100.015.6212925
TACACCG72850.014.5307555
CTATACT260500.014.4453294
TACACTG269100.014.11835
GTATAGA246600.014.1152791
TCTCCGC215200.014.0493145145
CTCCGAC108450.013.972627145
TCTATAC251450.013.8695143
TACACAG330800.013.3480135
CTTACAC213450.012.8399543
CTACACT212800.012.640674
GTATTAG242000.012.5257061
GTGTAGG161050.012.5177241
TAGACTG219050.012.24681955
CCTATAC154950.012.1192563
GTGTAGA217850.012.0501461
CTAACAC246700.012.0498753
GTATAGG146350.012.0407871
GTGTATA374350.011.8553651
Produced by FastQC (version 0.11.5)