FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_6852.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_6852.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24594560
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG252000.015.3067095
CTACACT141000.013.88410954
CTATACT188450.013.8509564
TACACTG165900.013.3735225
GTATTAG203650.012.7833931
TCTATAC209500.012.632323
TACACAG200100.012.5009425
TACACCG87650.012.4909585
CTAGGAC66300.012.3577443
GTATAGG117950.012.3576021
GTATAGA210450.012.19805051
CCTACAC119750.011.867383
TATACAG242000.011.864535
TATACCG71800.011.7139755
ATACTGT337300.011.521846
GTACTAG68400.011.4499541
TAGGACT132450.011.33161454
TAGACAG220950.011.2228415
TAGACCG50400.011.2210845
ATACCGT104700.011.2186796
Produced by FastQC (version 0.11.5)