FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_6214.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_6214.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32538058
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC58100.019.71674145
ATGCCGG151250.017.496517145
TCTCCGC144250.015.229335145
TATACTG342000.015.2008745
CACGAGC81350.014.527277145
CTACACT201100.014.0614484
CTATACT219700.013.993084
TACACTG270550.013.6677865
GTATTAG217400.013.2093841
GTGTAGG136200.013.1512421
CGTCTTG114050.013.09562145
GGACCGT22450.012.59586
CCTACAC141950.012.5653693
TAGTACT173250.012.5134024
CTTACAC199600.012.3507653
TATACAG294800.012.3221295
GTATAGA210450.012.301731
TCTATAC213800.012.2765513
TGCCGTG141200.012.272056145
CCACGAC76100.012.194934145
Produced by FastQC (version 0.11.5)