FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_3256.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_3256.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27460447
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG206500.015.3090245
TATACTG273750.014.8590085
GTATAGG113950.014.4457481
TACACCG67900.013.7752985
TATACCG57400.013.5161485
GTATAGA201550.013.2401691
CTACACT160950.013.1995084
TACACAG249550.013.1619945
GTGTAGG127050.013.0133371
CTATACT196250.012.9312184
GTGTAGA183100.012.7525211
CCTACAC133150.012.6335993
CTAGGAC93450.012.5694413
TAGACAG291650.012.4802365
GTATTAG200350.012.3784221
CTTACAC167850.012.1817043
GTACTAG80650.012.1383011
GTATAAG189450.012.0954211
TATACAG257150.012.0398865
CTAGTAC79900.011.8878893
Produced by FastQC (version 0.11.5)