FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30981.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30981.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29140546
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG23450.025.044146145
CACGAGC86150.016.495474145
TATACTG342250.016.333565
TCTCCGC93000.016.293985145
CTCCGAC38600.015.590312145
ATGCCGG100550.014.782089145
TACACTG267500.014.6636955
TACACCG23900.014.5617415
CTATACT212650.014.3543314
GTGTAGG128350.013.5592381
CTACACT193800.013.2438964
CTTACAC189650.013.22788053
GTATAGA206650.013.1938741
CTAGTAC92850.013.040723
GTATAGG113750.012.9409031
TCTATAC206950.012.8227933
CCTACAC134450.012.7806963
GTATTAG207500.012.7554171
TAGACAG402700.012.711395
ATACTGT445750.012.5898876
Produced by FastQC (version 0.11.5)