FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30978.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30978.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27225357
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG287050.015.3573725
TACACTG226350.015.0872925
TCTCCGC92600.014.876526145
GTATAGG109800.014.5952881
TACACCG39400.014.1698555
CTACACT172200.013.6843794
CTATACT212100.013.3321084
GTGTAGG121600.013.0597021
GTATAGA200850.012.9973081
CCTATAC111600.012.7990033
TCTATAC198800.012.6192583
TACACAG283150.012.3168435
TCTACAC176600.012.1938213
CTAGACT156450.012.142294
TAGACAG348650.012.1241065
TAGACTG187350.012.07457355
GTATAAG189850.011.9551911
CCTACAC127700.011.8666873
ATACTGT381900.011.790066
CTAGGAC114050.011.7611693
Produced by FastQC (version 0.11.5)