FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30908.TR4_batch_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30908.TR4_batch_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20095480
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG244000.017.0861385
TACACTG173650.016.4508135
GTATAGG75550.015.7415951
TATACCG19550.015.5764485
TCTATAC143900.014.8134683
ATGCCGG52850.014.404719145
CTACACT123750.014.2374924
GTGTAGG91150.014.081731
CTTACAC123500.013.7378623
CCTACAC90350.013.6424073
ATACTGT312550.013.5243726
GTATTAG142750.013.4619871
CTATACT145750.013.4315964
CTCCGAC39650.013.348737145
TACACCG25250.013.208775
GTCCTAG90350.013.0827461
GTATAGA143650.013.0242731
GTACTAG63150.012.97610951
CCTATAC79150.012.9163083
CTAGCAC101450.012.7214963
Produced by FastQC (version 0.11.5)