FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30908.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30908.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26535560
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG68100.016.395828145
TACACTG192500.015.0661425
TATACTG263650.014.9054245
ATGCCGG164550.014.848811145
CCTACAC127050.014.6664673
CTACACT150500.014.59740454
CTATACT189300.014.5929774
TACACCG73500.014.5011625
TCTATAC190950.013.7073843
GTATAGG109750.013.4790931
TACACAG220200.013.3684645
TAGACTG144350.012.758215
GTGTAGG127500.012.6263491
CTAGACT119950.012.5728184
CTTACAC152300.012.5681423
GTACTAG80400.012.1762261
TATACAG238350.012.1679555
GTATAGA194350.012.0891411
TAGACAG253500.011.9841945
CTACCCT120250.011.8179054
Produced by FastQC (version 0.11.5)