FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30907.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30907.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25989448
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG226400.015.2763345
TATACTG299100.015.0540155
CTACACT165900.014.3352834
CCTACAC121800.012.97738553
GTATAGG96750.012.9669891
TACACCG33550.012.9668915
TCTATAC181850.012.9583023
TACACAG275800.012.8818725
TCTCCGC95200.012.871012145
CTATACT186050.012.8606564
TAGACTG181250.012.7211485
CCTATAC98900.012.6098573
CTCCGAC45750.012.519845145
GTGTAGG117500.012.3434391
GTATAGA183400.012.2576271
TATACAG249050.012.2275645
CTTACAC157900.012.16863253
TCTACAC159250.012.1565363
CTAGCAC132750.012.125413
TAGACAG335050.011.9672035
Produced by FastQC (version 0.11.5)