FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30893.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30893.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25297996
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG109950.016.552295
TATACCG62750.015.1369085
TATACTG197250.014.7403215
CTATACT151400.014.080034
TACACTG151000.014.0692265
CTACACT127850.013.8379344
GTGTAGG123500.013.211491
GTATAGG94950.013.1361991
GTATTAG201550.012.952571
TCTATAC159950.012.5568113
AGGACCG67900.012.4938325
TACACAG171050.012.2505155
GTATAGA149500.012.22351
GTGTAAG118750.012.2132881
TAGACAG180650.012.1614195
GTATAAG148950.012.07389451
TCTCCGC163000.012.054239145
ATACCGT129850.012.0054326
CTACACC195700.012.0042874
GGACCGT60650.011.9550266
Produced by FastQC (version 0.11.5)