FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30889.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30889.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31416988
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG106900.014.6502965
TATACTG240050.014.4074465
GTATTAG192650.014.152621
CTATACT171800.014.0116324
TACACTG192950.013.8284455
GTATAGG106950.013.6958381
CTACACT153250.013.5312954
GTGTAGG132400.013.4730271
TCTCCGC227500.012.9390955145
TATACAG221500.012.9298565
TACACAG226800.012.8834545
TCTATAC175400.012.8145433
GTACTAG78650.012.723261
CTAGTAC83250.012.7156823
GTATAAG166350.012.466981
ATGCCGG186150.012.307883145
TAGACTG164550.012.2935325
CCTATAC111200.012.2581263
CTAGGAC113800.012.2329163
CCTACAC144850.012.1134523
Produced by FastQC (version 0.11.5)