FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30785.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30785.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30369164
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC82700.016.043713145
CTATACT216250.015.3226194
TACACCG69250.015.18149955
TATACTG315950.015.1457945
CTACACT175650.014.8190124
ATGCCGG169250.014.77916145
TACACTG226900.014.63515955
CCTACAC138850.014.5689543
GTATAGA221450.013.3936151
GTGTAAG175950.013.188971
TCTATAC225150.012.8812523
TACACAG270200.012.61185555
CTAGTAC81900.012.4826063
TCTCCGC164800.012.406555145
CTAGACT134100.012.111344
TAGACAG313450.012.0976195
GTATTAG214950.012.0442831
TATACAG269650.012.0191455
CTGTGCG78550.011.9985459
GTGTAGG137750.011.9504541
Produced by FastQC (version 0.11.5)