FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_30783.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_30783.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23638630
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG288850.015.5879585
TACACTG215250.014.4168595
GTATAGG98250.013.064161
GTGTAGG106200.012.9738791
CCTATAC94050.012.8743393
CTAGGAC109300.012.8691213
TATACCG24150.012.6096265
GTACTAG80600.012.5960331
CTATACT184850.012.5907874
CTTACAC165600.012.390633
ATACTGT354650.012.3688186
TCTATAC176650.012.2312243
TAGGACT144400.012.2013074
CTACACT163150.011.9989714
GTATAGA175900.011.996871
TACACAG263100.011.9877715
CCTAGAC114550.011.3931733
GTATAAG163950.011.3674351
CTAACAC180900.011.3426673
GTCCTAG111400.011.3267371
Produced by FastQC (version 0.11.5)