FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_2397.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_2397.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28881828
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG34100.019.773836145
TACACTG242900.014.2982095
CTACACT181800.014.0386044
TATACTG240050.013.8034665
CACGAGC91000.013.78374145
GTATAGG116100.013.2420581
CCTACAC133350.013.1581623
CTAGGAC131050.012.8911543
ATGCCGG134350.012.736107145
TACACCG40450.012.36813455
CTAAGAC159650.012.2621813
CTATACT188050.012.26108554
TCTCCGC105200.012.198877145
GTGTAGG130300.012.1885371
TCTATAC179950.012.0876613
CCTATAC115500.011.990143
CTAATAC216850.011.9700653
GTGTAAG182800.011.9410391
GTATAGA187950.011.9225191
CTAGACT157500.011.831174
Produced by FastQC (version 0.11.5)