FastQCFastQC Report
Tue 27 Aug 2019
H3FMKBGXC_n01_MG_2397.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3FMKBGXC_n01_MG_2397.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22360055
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG253800.015.655035
TACACTG171600.014.4079465
GTATAGG97850.014.3026921
TACACCG47250.013.9638315
TATACCG40300.013.3134825
GTATAGA170650.012.6203521
CCTACAC106400.012.6064883
GTATTAG172300.012.5836681
ATACTGT316900.012.4693586
GTACTAG74050.012.4365521
CTAGGAC89300.012.09758953
CCTATAC97700.012.0222153
CTATACT174500.011.9663874
TATACAG219950.011.9000345
GGACCGT27600.011.8215196
TCTATAC165600.011.6900123
CTAATAC181550.011.6214623
CTTACAC136900.011.5456143
CTAACAC156350.011.4541573
TAATACT287150.011.4381384
Produced by FastQC (version 0.11.5)