FastQCFastQC Report
Fri 18 Nov 2016
H3F7MBCXY_l02n01_c37_3.3510000006fae4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3F7MBCXY_l02n01_c37_3.3510000006fae4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31308575
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC583704818.643608021125203RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG10930903.4913438251341686Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG7638402.4397149982073603Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC5891651.8818007526691969RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC5144371.6431185386112273RNA PCR Primer, Index 6 (100% over 51bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC4790751.53017184589206No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG3249031.0377444517995469Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA2392000.7640079435106836No Hit
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA1975830.6310826985897634RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA1817620.5805502166738665RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA1407360.4495126335197306RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1225730.3914997728258153RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG1090260.34823047679429675No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA1007450.32178085396732364RNA PCR Primer, Index 6 (100% over 37bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT895740.2861005331606437No Hit
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC867100.2769528795226228RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG855830.2733532267118513No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC845200.26995799074215293RNA PCR Primer, Index 1 (100% over 24bp)
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT807970.25806667981535414RNA PCR Primer, Index 1 (100% over 30bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG782760.2500145726849593No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC719080.22967509699818658RNA PCR Primer, Index 6 (100% over 36bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC658570.21034812347735404RNA PCR Primer, Index 5 (97% over 35bp)
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA658050.21018203479398215RNA PCR Primer, Index 6 (100% over 37bp)
CGTAGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA584980.18684338076709017RNA PCR Primer, Index 6 (100% over 40bp)
TTTTTTATGACTGACAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA584600.18672200826770302RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA539960.1724639336028548RNA PCR Primer, Index 6 (100% over 37bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA537500.1716782063699801No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG512410.16366442739728654RNA PCR Primer, Index 6 (100% over 34bp)
GTTAAAACTCACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC475080.15174117633907006RNA PCR Primer, Index 6 (100% over 36bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG438710.1401245505424632No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA417040.13320312406425394RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG405470.12950765085922947Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA399560.12761998909244512No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG396960.1267895456755857No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG389170.12430140943814913No Hit
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC379790.12130542511117161RNA PCR Primer, Index 1 (100% over 33bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT371110.11853302170411781No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA366010.1169040750018166RNA PCR Primer, Index 1 (100% over 23bp)
CATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT357460.11417319376560574RNA PCR Primer, Index 1 (100% over 30bp)
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC355920.11368131574177362RNA PCR Primer, Index 1 (100% over 26bp)
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA352220.1124995308793198RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC351400.11223762180169491No Hit
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC337450.10778197346892984No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA326260.10420787276329249No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG326100.10415676855302422No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA319110.10192415336692903RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC2413500.044.946531
TACATCT6157000.044.868621
CACCGCT2388900.044.78462
CATCTTT6173950.044.7571643
ACATCTT6172850.044.75612
GCACTGC410050.044.704521
ATCTTTT6186650.044.667474
CTTTTTT6179550.044.6313936
TCTTTTT6197750.044.5874755
GCCCCAA219350.044.5453831
TACCCCG52100.044.52190814
TCCGACA967400.044.4008331
CGACGGG52150.044.39293719
CACTGCT408700.044.390232
CGCGATG6162100.044.34118316
TCGCGAT6164000.044.3377415
TGTGACG243750.044.332895
TAATCGC6221800.044.32722512
TTAATCG6223500.044.31728711
AATCGCG6207550.044.31336613
Produced by FastQC (version 0.11.2)