FastQCFastQC Report
Fri 18 Nov 2016
H3F7MBCXY_l02n01_c37_2.3510000006fad7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3F7MBCXY_l02n01_c37_2.3510000006fad7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27490373
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC457178216.630483696965477RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG12589694.579672309284417Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG8553383.1114092195111356Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC5813042.1145729816034144RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC5379041.9566995325963745No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG3655511.329741869999363Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA2235750.8132847087960575RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA2138750.7779996291792767RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA2033120.7395752687677246No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1501320.5461257291779926RNA PCR Primer, Index 1 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA1443220.5249910577786631RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG1420700.5167990990882517No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC1355360.49303077844742227RNA PCR Primer, Index 5 (100% over 51bp)
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1229460.4472329276870852RNA PCR Primer, Index 1 (100% over 24bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT1031670.37528410400251755No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA990920.36046073292639574No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG953430.3468232315363637No Hit
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC910120.33106862536932474RNA PCR Primer, Index 1 (100% over 31bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT798310.29039620524610565No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT722590.26285201732257324RNA PCR Primer, Index 1 (100% over 30bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG703440.25588594232606443No Hit
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG634890.23094994018451478RNA PCR Primer, Index 5 (100% over 37bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA625450.22751601078675796No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA575160.2092223339421404RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG524970.19096503346826177Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG524940.1909541205570401No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG513850.18691998104209062No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA509500.18533760891494633RNA PCR Primer, Index 1 (100% over 34bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC502140.18266030802855968RNA PCR Primer, Index 5 (100% over 35bp)
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC466480.16968849422305038RNA PCR Primer, Index 1 (100% over 26bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA456920.16621091318040682RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTATGACTGACAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA450210.16377005870382333RNA PCR Primer, Index 1 (100% over 32bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC427880.15564721511781598No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA405810.14761895009572987RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC393190.14302825210847447RNA PCR Primer, Index 1 (100% over 33bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA389900.14183146951116304RNA PCR Primer, Index 5 (100% over 36bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG377230.13722258333853818RNA PCR Primer, Index 5 (100% over 37bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC373540.13588029525827097No Hit
CATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT350870.12763377201175116RNA PCR Primer, Index 1 (100% over 30bp)
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG347580.12643698941443973No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG328710.11957276825600002No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG323220.11757570550243171No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA322180.11719739124674663RNA PCR Primer, Index 1 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA322150.11718647833552495RNA PCR Primer, Index 1 (100% over 23bp)
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG314220.11430183213592628RNA PCR Primer, Index 5 (100% over 37bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC306750.11158451724172676RNA PCR Primer, Index 5 (100% over 35bp)
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG291420.10600801960744585No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA279670.10173379604561932No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG276640.10063159201222915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC2691400.044.9805871
TACATCT4829900.044.866861
CACCGCT2654900.044.8011742
GCACTGC451050.044.7778551
CATCTTT4842400.044.7603843
ACATCTT4843350.044.7446332
CTTTTTT4845100.044.6495176
ATCTTTT4854400.044.647424
TCTTTTT4860400.044.5941545
TCCGACA1088200.044.5561261
TAATCGC4852100.044.5557112
CGCGATG4809300.044.55552716
TCGCGAT4810750.044.5500515
TTAATCG4853550.044.5493511
AATCGCG4843650.044.5354313
ATCGCGA4821300.044.5113614
TTTAATC4860150.044.49718510
GCCCCAA268800.044.473091
TGTGACG208700.044.4464455
TACCCCG47300.044.42588814
Produced by FastQC (version 0.11.2)