FastQCFastQC Report
Fri 18 Nov 2016
H3F7MBCXY_l02n01_c37_1.3510000006fabd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3F7MBCXY_l02n01_c37_1.3510000006fabd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39511865
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC579168614.658093208204676RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG13969393.5354924400556644Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG10320312.611952131340801Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC8436642.1352168519506733RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC7166301.8137083632979611No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC5365401.3579212218911965RNA PCR Primer, Index 4 (100% over 51bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG3950550.9998389091479231Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA3092830.782759811514845No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA2760900.6987521343272457RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA2552210.6459350881058132RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA2183820.5526998029579216RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1692240.42828654127057786RNA PCR Primer, Index 1 (100% over 25bp)
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1417120.3586568237161167RNA PCR Primer, Index 1 (100% over 31bp)
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG1312540.33218882479984174No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT1276860.32315862589629724No Hit
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1265040.3201671194209638RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG1182890.29937589632886225No Hit
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG1062320.2688610117492556RNA PCR Primer, Index 2 (97% over 35bp)
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC1001870.253561809851294RNA PCR Primer, Index 4 (100% over 37bp)
ACATCAGTACTGGGCTGGGCGACTTCCCGGGAACGTGCGGTGCTTGCTTCT970100.24552118711683185No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG956680.24212473898663098No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC945780.23936607396284637RNA PCR Primer, Index 1 (100% over 24bp)
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC936000.23689086809746898RNA PCR Primer, Index 4 (100% over 37bp)
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT927820.23482060388695902RNA PCR Primer, Index 3 (100% over 34bp)
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA903100.22856425531925664RNA PCR Primer, Index 2 (97% over 36bp)
CGTAGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA810980.20524973954026215RNA PCR Primer, Index 4 (100% over 40bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA783660.1983353607834001No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA778740.19709016519468264No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG756020.19133999369556462No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA722010.182732452644288RNA PCR Primer, Index 1 (100% over 23bp)
ATCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC641450.16234364032171097RNA PCR Primer, Index 1 (100% over 33bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA607840.1538373346841512No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA606450.1534855416214851RNA PCR Primer, Index 1 (100% over 23bp)
TTTTTTATGACTGACAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA589420.14917544388248946RNA PCR Primer, Index 1 (100% over 32bp)
GTTAAAACTCACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA531560.13453174128834466RNA PCR Primer, Index 2 (97% over 36bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG530220.13419260265239316No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG517780.1310441812857986No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG508100.1285942842738504Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG494720.1252079596850212No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC487290.12332751187523039RNA PCR Primer, Index 1 (100% over 26bp)
CATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGT486040.12301115120736518RNA PCR Primer, Index 1 (100% over 30bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA471770.11939957782301595RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGCTTAGCTCAATGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTCC468670.11861500336671023RNA PCR Primer, Index 1 (100% over 27bp)
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC459820.11637516983822455No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA458830.11612461218927529RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC452760.11458836478612183RNA PCR Primer, Index 4 (100% over 37bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA450270.11395817433573432RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGTGGAATTCTCGGGT447220.11318625430614324No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG431680.10925325848324295No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG402550.10188078947931209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC3201400.044.9691431
TACATCT6168200.044.8483661
CACCGCT3170500.044.787492
CATCTTT6184900.044.745153
GCACTGC493750.044.72521
ACATCTT6186400.044.72492
ATCTTTT6204400.044.605614
CTTTTTT6198450.044.5667426
CGACGGG66500.044.5569719
TCCGACA1361250.044.5506631
TCTTTTT6216400.044.5285535
GCCCCAA255500.044.4090921
TCCGATA463300.044.404811
TACCCCG67000.044.35877214
TCGCGAT6157300.044.3339615
TAATCGC6229400.044.324312
CCCCAAT253450.044.3231432
CGCGATG6157350.044.3215516
TTAATCG6230750.044.3193911
CCGACAT1350950.044.3138662
Produced by FastQC (version 0.11.2)