FastQCFastQC Report
Fri 18 Nov 2016
H3F7MBCXY_l01n01_sd2_3.3510000006fb1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3F7MBCXY_l01n01_sd2_3.3510000006fb1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24035130
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC296315112.328416779938365RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG21787719.0649436886757Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC10260524.268967964808178RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG7766013.2311079657151844Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA4669121.9426231520278856RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA3576891.4881924915737923RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT3087831.2847153312671908RNA PCR Primer, Index 1 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA2176490.905545341339947RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC1976450.8223171665807507No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG1500150.6241489020446322No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC1070340.445323158227145RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG972400.40457447078505504No Hit
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA736410.30638902306748494No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA728160.30295654735381083RNA PCR Primer, Index 1 (100% over 22bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG707520.2943691172046916No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC684220.2846749736739514No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG661330.27515141378473923Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG637580.26527004430598045No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT633880.2637306309556054No Hit
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA631520.2627487348726635RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG614010.2554635652064291No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT553760.23039609105505152RNA PCR Primer, Index 9 (100% over 36bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA553570.23031704009922144RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA547940.22797463546067775No Hit
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC547370.22773748259318755No Hit
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA543440.2261023759804919No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA499160.20767934269546284RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG470750.195859144510556Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT463010.1926388582046363RNA PCR Primer, Index 1 (100% over 30bp)
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC454150.18895258731698145RNA PCR Primer, Index 1 (100% over 26bp)
ACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATT430340.17904625437848684No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG415680.1729468490497035No Hit
CTCATAATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAAT408060.1697764896632554No Hit
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA406060.16894437433872836RNA PCR Primer, Index 7 (97% over 35bp)
ATGGGTGTGACAGTACTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC400860.16678087449495801RNA PCR Primer, Index 1 (100% over 31bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG393950.16390591604871702No Hit
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG370990.15435323212314642No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG368160.15317578893894063No Hit
ACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCC336850.14014902353346956No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA331870.1380770563753972No Hit
TCGGTCATAGTGTAATGGTTATCATTTAAGATGGAATTCTCGGGTGCCAAG320910.1335170643969889No Hit
CGCGCAGCTACGCCGTCTGGGATAATGGCTGAAAGCCTTGGAATTCTCGGG318210.13239370870887737No Hit
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG312590.13005546464695633No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG310660.12925247335878776No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC308380.1283038618888269No Hit
CTAGAGGAAGGAGAAGTCGTAACAAGGTAACGGTATGGAATTCTCGGGTGC307190.1278087532707333No Hit
GGGTGAGTAGTCTAGTGGTATGATTCTGGAATTCTCGGGTGCCAAGGAACT295240.12283686420668415RNA PCR Primer, Index 1 (100% over 25bp)
TCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGG294090.12235839789508107No Hit
ATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGGG288120.1198745336513678No Hit
GGTTCAACTCCGGTCGTAGGCACCATGGAATTCTCGGGTGCCAAGGAACTC277270.11536030801580853RNA PCR Primer, Index 1 (100% over 26bp)
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC273140.11364198987066015RNA PCR Primer, Index 1 (100% over 24bp)
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG271790.1130803120266044No Hit
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG267010.11109155640098471RNA PCR Primer, Index 6 (100% over 34bp)
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT266920.11105411121138101No Hit
TAAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTC266560.11090433045296613No Hit
GGGTGAGTAGTCTAGTGGTATGATTCCTGTGGAATTCTCGGGTGCCAAGGA264120.10988914975704313RNA PCR Primer, Index 1 (100% over 22bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCCTGGAATTCTCGGGTGCCAAG263120.10947309209477961No Hit
CATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGG257810.10726382590816026No Hit
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG251660.10470507128523956Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGACATCGTTCATCGGTTAGGATAATGGAATTCTCGGGTGCCAAGGAAC247950.10316149735824189RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC246910.10272879738948781RNA PCR Primer, Index 9 (100% over 51bp)
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA243270.10121434749884857RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC4793500.044.9909741
CACCGCT4705950.044.8860472
TACATCT3209650.044.8648721
ACATCTT3209300.044.830932
CATCTTT3210250.044.8204653
CCCCAAT318650.044.780182
ATCTTTT3214050.044.756974
CTTTTTT3205050.044.7499286
GCCCCAA324300.044.7262881
TCCGATA366300.044.7174951
TCCGACA1119300.044.696951
TCTTTTT3216800.044.6907235
ACCGCTG4730350.044.647383
CGCGATG3183900.044.6057616
TAATCGC3217500.044.60185612
TCGCGAT3184850.044.5992415
TTAATCG3217350.044.5878511
AATCGCG3214650.044.5812113
ATCGCGA3191300.044.57254814
TTTAATC3222450.044.5382310
Produced by FastQC (version 0.11.2)