FastQCFastQC Report
Fri 18 Nov 2016
H3F7MBCXY_l01n01_sd2_2.3510000006faf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3F7MBCXY_l01n01_sd2_2.3510000006faf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14125354
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG12411948.786993940116474Illumina Small RNA Adapter 2 (100% over 21bp)
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC6709324.74984202165836RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC6593644.667946728981094RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG4792973.393168057947433Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA2430701.7208064307627262RNA PCR Primer, Index 1 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA1941841.3747195291530392RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC1634031.1568064064093544No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA1452001.0279388396212938RNA PCR Primer, Index 1 (100% over 22bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG1391260.9849381473908547No Hit
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT1370300.970099581221115RNA PCR Primer, Index 1 (100% over 25bp)
ACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATT714500.5058280309293488No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGTG703240.497856549294269No Hit
ACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCC626400.44345791263001266No Hit
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT574600.406786265321209No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG523870.3708721211517955No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG519320.3676509629422385No Hit
ATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGG506180.3583485412117813Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA434380.3075179567181113No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG408640.28929540456118835No Hit
AGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAA369460.26155804661603527RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA359750.2546838826127827RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTGTGACGATATTAAATGCACCACCTAGCTGATTTACAGATGATTGCA342550.24250719663379763No Hit
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG339160.2401072567809628No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC338930.23994442900333685No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA334800.23702060847466197RNA PCR Primer, Index 1 (100% over 23bp)
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA327090.23156233818989597RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCAGCTACGCCGTCTGGGATAATGGCTGAAAGCCTTGGAATTCTCGGG326590.23120836476027432No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC310260.21964759254883098RNA PCR Primer, Index 1 (100% over 24bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC286120.20255775536669735No Hit
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG269630.1908837116577751Illumina Small RNA Adapter 2 (100% over 21bp)
ATCTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGG267160.1891350829154441No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA259360.18361309741334625RNA PCR Primer, Index 1 (100% over 22bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG259310.1835777000703841No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG249840.17687344331334987No Hit
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC240100.16997804090431998RNA PCR Primer, Index 1 (100% over 26bp)
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT234910.16630379670484718RNA PCR Primer, Index 1 (100% over 30bp)
ATGGTGCGTAGATTCTGGGCGCGAGCATGCGCTTCATGGAATTCTCGGGTG232590.1646613599914027No Hit
ATGTTGGACCGATACGAATAATCATACTCAAAAGTGGAATTCTCGGGTGCC228820.1619924003320554No Hit
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG228700.1619074467089462No Hit
GATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAG210920.14932015155160006No Hit
GCTGGAGCGGGGTTCGACTCCCCGTGTCGGAACCATGGAATTCTCGGGTGC205140.1452282187051737No Hit
GCGGAGGAAAAGAAACTAACTAGGATTCCCTTAGTAAAGGTGGAATTCTCG202300.14321764962492267No Hit
ATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGGG201630.14274332522922964No Hit
GCCCCAATAGTATATCGGTTAGAACACTGGAATTCTCGGGTGCCAAGGAAC186950.1323506653355378RNA PCR Primer, Index 1 (100% over 24bp)
ATCCCGTGGATTTATCCGCGTGGGTTCGAATCCCATGGTCGTCGCCATGGA183550.12994364601411051No Hit
CTGGAAGTCGTGTGTTCGATCCACACGGGAGGAGCCATGGAATTCTCGGGT177350.12555437548680196No Hit
GCGGGGAAAGAAGACCCTGTAGAGCTTTGGAATTCTCGGGTGCCAAGGAAC173430.12277922379856816RNA PCR Primer, Index 1 (100% over 24bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA166640.11797226462430606No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGATGGAATTCTCGGGTGCCAA166240.11768908588060874No Hit
CTTGGGCTCTGGGGGAACTACGACCGCAAGGCTTGGAATTCTCGGGTGCCA165030.11683247018092432No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA164710.11660592718596645RNA PCR Primer, Index 1 (100% over 23bp)
ATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGTG164520.11647141728271024No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGTGGAATTCTCGGGTGCCAAGGAA163720.11590505979531557RNA PCR Primer, Index 1 (100% over 23bp)
CATGACAGGTTAATCTTTGAATGCAAATTGCGCTAAACTTGGAATTCTCGG159870.11317946438722881No Hit
GCACCGCCGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG157990.11184852429185137Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAACTCC156100.11051050472788151RNA PCR Primer, Index 1 (100% over 27bp)
ATCCTAAGGTCGCGCGTTCGAGTCCCGCCGAAAGCACCATGGAATTCTCGG153670.10879019385992025No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGTGGAATTCTCGGGTGCCAAGG150870.10680794265403898Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGATGGAAGGCCAGTTCGATTCTGGCTTGGGGCACCATGGAATTCTCGG150470.10652476391034166No Hit
AAGGAGAAGACTGTGGGTTCAAGTCCCACCATGGCTGCCATGGAATTCTCG148300.10498851922578366No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC148170.10489648613408202RNA PCR Primer, Index 8 (100% over 51bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGTGGAATTCTCGGGTGCCAAG145010.10265937405887315No Hit
CAATGAGAGCCATCGGGGGTAGATCTATCTTGGAATTCTCGGGTGCCAAGG143090.10130011608912598Illumina Small RNA Adapter 2 (100% over 21bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCTGGAATTCTCGGGTGCCAAGG142530.10090366584794973Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGC2687450.044.9868661
CACCGCT2657700.044.9057852
CGACGGG10100.044.77332319
TCCGATA247150.044.7521321
TCCGACA660850.044.7358321
CCCCAAT133150.044.6744272
ACCGCTG2671550.044.669613
CATCTTT745800.044.6563953
TACATCT747350.044.639821
ACATCTT746550.044.629622
GCCCCAA134900.044.6248781
TAATCGC743350.044.56916812
CCCCGAC10250.044.55708716
CTTTTTT745550.044.553646
CGCGATG733100.044.54801616
TCGCGAT733650.044.54512815
CCGACAT655050.044.541782
AATCGCG741700.044.52878613
ATCGCGA737300.044.51379414
TTAATCG744450.044.50935411
Produced by FastQC (version 0.11.2)