FastQCFastQC Report
Fri 18 Nov 2016
H3F7MBCXY_l01n01_sd2_1.3510000006faca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3F7MBCXY_l01n01_sd2_1.3510000006faca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10728105
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACATCTTTTTTAATCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC6177835.758547292368969RNA PCR Primer, Index 1 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTGGAATTCTCGGGTGCCAAGG4892374.560330086254749Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCTGGAATTCTCGGGTGCCAAGGAAC3234623.015089803837677RNA PCR Primer, Index 1 (100% over 24bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGTGGAATTCTCGGGTGCCAAGG1829031.7048956922028635Illumina Small RNA Adapter 2 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCATGGAATTCTCGGGTGCCAAGGAA1152301.0740946327426886RNA PCR Primer, Index 1 (100% over 23bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTTGGAATTCTCGGGTGCCAAG1068790.9962523670303376No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACTGGAATTCTCGGGTGCCAAGGA1032200.962145691154216RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTTGGAATTCTCGGGTGCCAAGGAACT759320.7077857645875018RNA PCR Primer, Index 1 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATGTTGGAATTCTCGGGTGCCAAGGA685130.6386309604538732RNA PCR Primer, Index 1 (100% over 22bp)
CGTAGTCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA610870.5694109071452973RNA PCR Primer, Index 7 (100% over 40bp)
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTGGC437430.40774209424684044No Hit
CCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG361540.3370026672930587RNA PCR Primer, Index 7 (100% over 36bp)
ACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCC321580.299754709708751No Hit
GCCCCAATAGTATATCGGTTAGAACACATCCTGGAATTCTCGGGTGCCAAG289340.26970280399008023No Hit
GGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA286930.2674563681097454RNA PCR Primer, Index 7 (100% over 37bp)
ATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGG274600.25596319200828105Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA249660.23271584310556245RNA PCR Primer, Index 7 (100% over 35bp)
ACTGAGCATTACTGATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATT231060.21537820519094472No Hit
CAGGTTCAAGTCCTGGACGGCGCGCCATGGAATTCTCGGGTGCCAAGGAAC229530.21395204465280682RNA PCR Primer, Index 1 (100% over 24bp)
CCGACCATAGCTCAGTTGGAAGAGCATGGTGGAATTCTCGGGTGCCAAGGA222290.2072034157010954RNA PCR Primer, Index 1 (100% over 22bp)
GTTAAAACTCACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG220340.20538576011327256RNA PCR Primer, Index 7 (100% over 36bp)
GATGTTGGACCGATACGAATAATCATACTCAAAAGGTTGGAATTCTCGGGT213560.1990659114540732No Hit
AGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT208020.193901905322515RNA PCR Primer, Index 7 (100% over 46bp)
AGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAGGAA198720.18523308636520616RNA PCR Primer, Index 1 (100% over 23bp)
ATCCTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGG187010.17431783152756242No Hit
ATCACAAGGAATAGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC186580.17391701516717073RNA PCR Primer, Index 1 (100% over 33bp)
GCTTTCCTAGCTCAACAGGATAGAGCTTTGGATGGAATTCTCGGGTGCCAA178490.1663760748053827No Hit
GATAGGACCCGAAAGGTGGTGAACTTTGCCTTGGAATTCTCGGGTGCCAAG175620.16370085863253575No Hit
CATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA173490.16171541945199083RNA PCR Primer, Index 7 (100% over 37bp)
TCCGACATCGTTCATCGGTTAGGATAAGTGGAATTCTCGGGTGCCAAGGAA166370.1550786462287608RNA PCR Primer, Index 1 (100% over 23bp)
ATGGTGCGTAGATTCTGGGCGCGAGCATGCGCTTCATGGAATTCTCGGGTG166210.15492950525745228No Hit
CGGGGTCACTGGTTCAAATCCGGTTGGTCGGACCATGGAATTCTCGGGTGC161130.15019427941840613No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTGGAATTCTCGGGTGCCAAGGAA159230.14842323038411723RNA PCR Primer, Index 1 (100% over 23bp)
AAGGGTCGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC157580.14688521411749791RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGTGGAATTCTCGGGTGCCAAG157150.14648439775710623No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCTGGAATTCTCGGGTGCCAA156100.14550566013289393No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTTGGAATTCTCGGGTGCCAAGGAA155690.14512348639391578RNA PCR Primer, Index 1 (100% over 23bp)
GCCCCAATAGTATATCGGTTAGAACACATCTGGAATTCTCGGGTGCCAAGG153420.1430075488634759Illumina Small RNA Adapter 2 (100% over 21bp)
CAATGAGAGCCATCGGGGGTAGATCTATCTTGGAATTCTCGGGTGCCAAGG152910.14253216201742994Illumina Small RNA Adapter 2 (100% over 21bp)
AAGTCTCTAAGCAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA152460.14211270303562465RNA PCR Primer, Index 7 (100% over 37bp)
TCCCATGGTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC151900.14159070963604478RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC144290.13449719218818235RNA PCR Primer, Index 7 (100% over 51bp)
TAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTGC141440.13184061863674898No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCTGGAATTCTCGGGTGCCAAGGA141180.13159826455837262RNA PCR Primer, Index 1 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCTTGGAATTCTCGGGTGCCAAG137360.12803752386838124No Hit
TCCGACATCGTTCATCGGTTAGGATAAGCGGTGGAATTCTCGGGTGCCAAG134920.125763124055926No Hit
CCGTGTCGGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA133950.12485895691736798RNA PCR Primer, Index 7 (100% over 37bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT126820.11821286238343119RNA PCR Primer, Index 7 (100% over 46bp)
CCTTGACACGGATCAGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT125720.11718751820568499RNA PCR Primer, Index 1 (100% over 30bp)
CTAAGGTCGCGAGTTCGAGCCTCACCGAAAGCACCATGGAATTCTCGGGTG123420.11504361674312472No Hit
GCGGGGAAAGAAGACCCTGTAGAGCTTTGGAATTCTCGGGTGCCAAGGAAC122130.11384116766194961RNA PCR Primer, Index 1 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC121630.11337510212661042RNA PCR Primer, Index 1 (100% over 26bp)
CTAAGTCAGATGGAATGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA116910.10897544347300851RNA PCR Primer, Index 1 (100% over 32bp)
CTAGAGGAAGGAGAAGTCGTAACAAGGTAACGGTATGGAATTCTCGGGTGC116390.10849073531625575No Hit
CCAACCGCAAGCTTACGATGGACTGGAATTCTCGGGTGCCAAGGAACTCCA115090.10727896492437389RNA PCR Primer, Index 1 (100% over 28bp)
CTTTTGGTACTGTGGATAGGGGTACGGTTTTCCACCGTTGGAATTCTCGGG114180.10643072565005655No Hit
AAGTCTCTAAGCAATGGATGTCTTGGCTCCTCACACGATGAAGAACGTTGG112280.10465967661576765No Hit
ATGTTGGACCGATACGAATAATCATACTCAAAAGTGGAATTCTCGGGTGCC109220.10180735553949184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTA650.045.025041
CCGCTAG253.8871716E-545.01144
GCTAGTC253.8871716E-545.01146
CGACGGG1700.044.99670819
GCACCGC1216050.044.989861
CACCGCT1199600.044.9157142
TACATCT691150.044.8621751
CATCTTT690500.044.848433
TCCGACA271850.044.826291
ACATCTT690650.044.8256532
GCCCCAA72550.044.7767941
TCCGATA112650.044.7052881
CTTTTTT691200.044.698826
ATCTTTT692900.044.6865924
ACCGCTG1206500.044.651383
TCTTTTT693650.044.6252945
CCGACAT269000.044.618172
TCGCGAT687900.044.4558215
TAATCGC695800.044.43931212
CGCGATG688300.044.4175316
Produced by FastQC (version 0.11.2)