FastQCFastQC Report
Fri 16 Aug 2024
H3CLFAFX7_n01_3084_Spikein_Input_13Aug24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CLFAFX7_n01_3084_Spikein_Input_13Aug24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7706156
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG796161.0331480442389176TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG201130.2609991284889639TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGGTGG149270.19370228165637965TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC138870.1802065777022941TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGGGG107810.13990113877788096TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTTG93670.12155217205569158TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGTTGG86860.11271508129344902TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGG82300.10679773417511922TruSeq Adapter, Index 7 (97% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)