FastQCFastQC Report
Fri 16 Aug 2024
H3CLFAFX7_n01_3084_Spikein_ChIP_13Aug24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CLFAFX7_n01_3084_Spikein_ChIP_13Aug24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4436567
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGG483961.0908434381809178TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTGG114810.2587811702156194TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATGG69240.15606661637252406TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGG56180.1266294411872964TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGGG55200.12442052605088574TruSeq Adapter, Index 10 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC54510.12286526947524966TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGG51270.1155623255548716TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGC45620.10282725359495304TruSeq Adapter, Index 10 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)