FastQCFastQC Report
Fri 16 Aug 2024
H3CLFAFX7_n01_2861_Spikein_ChIP_Rep1_13Aug24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CLFAFX7_n01_2861_Spikein_ChIP_Rep1_13Aug24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5159766
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC225900.4378105518738641TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTGG172550.33441439010993906TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTGG154740.2998973209250187TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGGTGG80330.15568535472345063TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGG76930.14909590861290994TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC70860.13733180923320942TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTGG70480.1365953417267372TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTGG66980.12981208837765124TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC63460.12299007358085617TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC54240.10512104618697826TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)