FastQCFastQC Report
Fri 16 Aug 2024
H3CLFAFX7_n01_12894_Spikein_Input_13Aug24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CLFAFX7_n01_12894_Spikein_Input_13Aug24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7149987
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5819448.139091721425508TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGG277260.3877769288251853TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGC203630.28479772061124026TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTC165870.2319864357795336TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATGC139380.19493741736873088TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC138460.19365070174253463TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTGC128310.17945487173613042TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGG97370.13618206578557415TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTGG83090.11621000150070203TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)