FastQCFastQC Report
Fri 16 Aug 2024
H3CLFAFX7_n01_12894_Spikein_ChIP_13Aug24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CLFAFX7_n01_12894_Spikein_ChIP_13Aug24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7213198
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC167659923.24349061262425TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC215030.29810633230919215TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC112210.1555620683086753TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC104940.1454833209902182TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGGATGC77100.10688740278583785TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC72550.1005795210390731TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)