FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10624470
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA22800.040.0035770-71
GTGTAGA14950.039.5020970-71
AGTGTAG18650.039.405670-71
CGAGTGT24100.035.1229970-71
ACGAGTG29950.032.19064768-69
TGTAGAT18750.019.9476870-71
GTAGATC11300.018.5819170-71
GTAGACC13800.014.3757173
GTCTAGA7450.014.0759761
GACGAGT67950.013.51939870-71
CGACGAG78150.013.51810170-71
TCTAGAC7150.013.4766973
TAGACTG6400.013.2835145
GTATAGG10800.013.1213741
CACTGAG8850.012.8007347
TAGACGG3556.511982E-1012.772135
TAGACAG7950.012.4759215
CCGACGA88050.012.29626870-71
GCCGACG94150.012.14189168-69
GTCCAGG12550.011.9692031
Produced by FastQC (version 0.11.5)