FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_RLW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_RLW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24553915
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA169550.043.9918870-71
AGTGTAG208550.041.6345970-71
GAGTGTA254600.038.03568670-71
CGAGTGT277850.034.9558670-71
ACGAGTG346300.028.68338868-69
TGTAGAT172250.028.4586570-71
GACGAGT389400.025.98511170-71
GTAGATC112300.024.44861270-71
CGACGAG532650.021.93911670-71
CCGACGA597400.021.53361570-71
GCCGACG746600.018.96181770-71
TGCCGAC811200.018.62934570-71
CTGCCGA974650.015.48170770-71
TGACGCC77250.014.2165970-71
TACACCG47750.014.1287125
TAGACGG23450.014.073425
CCTAGAC54350.013.5963433
GCTGCCG1170200.013.43735768-69
GTATAGG68800.013.4161021
TATACCG55200.013.3329265
Produced by FastQC (version 0.11.5)