FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_RLS-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_RLS-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25030142
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG168900.038.62320770-71
GTGTAGA147300.038.4259770-71
GAGTGTA195450.036.36032570-71
CGAGTGT214350.033.42103670-71
ACGAGTG272750.027.04065970-71
TGTAGAT151900.025.25556670-71
GACGAGT304450.024.46925570-71
GTAGATC105450.022.60902270-71
CCGACGA495700.021.037770-71
CGACGAG469000.019.00490270-71
GCCGACG632850.017.6438770-71
TGCCGAC649150.017.46505770-71
CTGCCGA772600.015.24420770-71
GTATAGG86500.015.230611
TATACCG62850.012.7795785
GTCTAGG51000.012.5438481
GCTGCCG954300.012.49480168-69
CTATACT93150.012.4799224
TATACTG76750.012.2156275
TACACCG55250.012.1057675
Produced by FastQC (version 0.11.5)