FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_RHW-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_RHW-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17660393
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA134150.044.2362970-71
AGTGTAG157050.041.85531270-71
GAGTGTA193100.036.5825970-71
CGAGTGT214800.035.09171770-71
TGTAGAT129900.030.74857370-71
ACGAGTG265450.028.63238770-71
GTAGATC88400.026.72293570-71
GACGAGT304400.026.03716370-71
CGACGAG416450.023.0187970-71
CCGACGA468900.022.34233370-71
GCCGACG593900.018.89848970-71
TGCCGAC636200.017.6150570-71
TAGACGG19200.016.8907325
CTGCCGA755250.015.32396768-69
GTCTAGG44350.014.8904531
CCTAGAC41450.014.5214083
GTATAGG50100.014.4062971
TACACCG35500.013.9909515
TATACCG41400.013.4790625
TATACTG63550.013.3783995
Produced by FastQC (version 0.11.5)