FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_FLW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_FLW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19667829
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA248800.052.2941770-71
AGTGTAG317750.047.42518270-71
GAGTGTA400150.042.1445570-71
TGTAGAT202300.040.24960770-71
CGAGTGT472100.036.63249670-71
GTAGATC138300.031.88404370-71
ACGAGTG578900.030.02282770-71
GACGAGT663050.026.99763968-69
CGACGAG847300.022.70515670-71
CCGACGA962050.020.15789470-71
AGATCTC91150.017.99196270-71
GCCGACG1156900.017.14513468-69
TGCCGAC1282400.015.64254568-69
TCGGTGG51900.014.58397170-71
ATCTCGG70750.014.26446270-71
TATACCG41600.013.8988785
TGACGCC80400.013.69349870-71
CTAGACA49400.013.6557674
CTCGGTG57750.013.60309670-71
CTGCCGA1511500.013.50641268-69
Produced by FastQC (version 0.11.5)