FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_FHUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_FHUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27700734
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA174350.044.8631470-71
AGTGTAG218900.044.14499770-71
GAGTGTA270400.041.61960670-71
CGAGTGT312500.037.69101370-71
ACGAGTG391900.029.57905668-69
GACGAGT432500.028.13232868-69
TGTAGAT177600.025.52005870-71
CGACGAG596300.021.98976170-71
CCGACGA658300.020.84537770-71
GTAGATC127100.020.36422270-71
GCCGACG817950.017.55037770-71
TGCCGAC873050.016.72353270-71
CTGCCGA1057000.014.42261270-71
TAGACGG31100.013.9977235
CCTAGAC65300.013.5134193
CTAGACA83850.013.2762234
GCTGCCG1285350.012.60993970-71
GTCTAGG63300.012.5101391
GTCTATG74550.012.0333721
TGACGCC93450.011.83531970-71
Produced by FastQC (version 0.11.5)