FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_FFS-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_FFS-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24879086
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG169500.043.06813470-71
GTGTAGA136100.041.7272970-71
GAGTGTA204250.040.60305870-71
CGAGTGT237200.038.4759270-71
ACGAGTG309000.030.18205570-71
GACGAGT331850.028.32573968-69
TGTAGAT154300.022.63813870-71
CGACGAG456600.022.3754870-71
CCGACGA510350.022.07670470-71
GTAGATC104650.020.4525470-71
GCCGACG638500.019.52301870-71
TGCCGAC691850.018.57854370-71
CTGCCGA842050.015.55607670-71
GTCTAGG55050.015.0721771
TAGACGG23850.014.4523875
CCTAGAC55100.013.8381393
GCTGCCG989900.013.54339168-69
GTCTATG66300.013.1316011
TATACCG67150.013.0938225
TAGAGCG55250.012.7946695
Produced by FastQC (version 0.11.5)