FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_FFLW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_FFLW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27263280
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA192800.050.31638370-71
AGTGTAG248800.047.70952670-71
GAGTGTA308600.044.19517570-71
CGAGTGT364550.039.13919470-71
ACGAGTG461150.030.72947370-71
TGTAGAT197400.030.5300870-71
GACGAGT506800.029.08121768-69
GTAGATC135450.025.18337870-71
CGACGAG647300.024.46528470-71
CCGACGA741100.022.69705470-71
TAGACGG25650.022.428095
GCCGACG902000.019.86658570-71
TGCCGAC1031550.018.1594470-71
GTACCGT35800.017.5299226
GTCTAGG62050.017.3214421
TGACGCC69350.016.75314370-71
CCTAGAC60300.016.2733463
GACGGTC28250.016.1187277
CTGCCGA1247650.015.50954870-71
TATACCG73600.015.1961785
Produced by FastQC (version 0.11.5)