FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n02_FFHUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n02_FFHUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29892905
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA157400.047.54260370-71
AGTGTAG199400.046.2288270-71
GAGTGTA245650.043.86467770-71
CGAGTGT293350.039.40599470-71
ACGAGTG387150.030.74590970-71
GACGAGT410150.029.51035170-71
CGACGAG547650.026.03204770-71
CCGACGA623850.024.5502470-71
TGTAGAT183600.024.417570-71
TAGACGG30200.022.9158295
GCCGACG763150.021.05182870-71
TGCCGAC872500.019.20087470-71
GTAGATC124300.018.79321970-71
GTCTAGG71550.018.0413911
GTACCGT41200.017.9943086
GACGGTC34250.017.1290847
CCTAGAC68600.016.6025713
CTGCCGA1078450.016.20835370-71
AGTACCG59900.015.6484885
ATAGACG38450.015.3416384
Produced by FastQC (version 0.11.5)