FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10624470
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG3650.021.2570085
TACACCG7100.020.7630065
GTGTATG12200.018.4468861
TCTAGAC8150.017.6122673
TAGACAG10600.017.201135
GTCTATG8200.016.5618611
CATAGAC10300.016.1957993
CTGTGCA6550.015.9904589
TACACGG4900.015.8343015
TATACTG13100.015.6952975
TAGCACG7000.015.5178354
TAGACTG7000.015.5176155
GGACCGT6550.015.3991596
CCTATGC9450.015.1894153
TCTCTAG10350.014.621071
TGGACCG10900.014.5922535
TACACAG8850.014.4655735
GTGTACA9950.014.4289111
CTATACT13800.014.3371294
CACTGTG7050.014.3061467
Produced by FastQC (version 0.11.5)