FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_RLW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_RLW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24553915
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT449350.023.21321794-95
CTTCTGC578950.022.02007594-95
TCTGCTT350700.021.62127194-95
TAGACGG27600.019.582555
TCTTCTG722700.019.39006894-95
TACACCG54950.018.99595
TATACCG68750.017.8834235
GTCTAGG68850.017.8615231
GTCTTCT813400.017.31919594-95
CTGCTTG240050.016.9505194-95
GTGTATG103700.015.8781641
GTATAGG83900.015.5428721
TACACAG65650.015.2713925
ACTGAGC73650.015.2359838
TATACTG107100.015.1779745
CGTCTTC952900.015.00038292-93
GTACCGT42750.014.95900256
AGTACCG52700.014.87476355
ACTGTGC66500.014.8269098
CCGTCTT1060450.014.67142394-95
Produced by FastQC (version 0.11.5)