FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_RLS-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_RLS-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25030142
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT386000.020.50268294-95
CTTCTGC478500.019.69889594-95
TCTGCTT313950.018.56229694-95
GTATAGG112250.017.1705321
TCTTCTG577350.017.11178294-95
TATACCG74650.016.0298185
CTATACT116000.016.0073744
GTCTTCT643450.015.61880194-95
TATACTG93550.014.9525855
CCTAGAC63000.014.8679443
CTGCTTG228950.014.53984594-95
GTCTAGG61000.014.4789531
ACTGTGC75350.014.0728638
CCTATAC136250.013.9311493
CCGTCTT809900.013.92260794-95
TAGACGG32900.013.9215465
GTCTATG74950.013.8215051
TGCCGTC1131450.013.80940794-95
GCCGTCT945650.013.70299394-95
ACTGAGC97800.013.6690648
Produced by FastQC (version 0.11.5)