FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_RHW-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_RHW-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17660393
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT347700.023.71543794-95
CTTCTGC443250.022.155294-95
TCTGCTT273400.021.20575794-95
TACACCG41000.019.4306355
TCTTCTG543150.019.107294-95
CTGCTTG186300.017.71332294-95
GTCTTCT627050.017.38758994-95
TAGACGG21950.017.3008275
TATACCG48150.016.8882065
TACACAG46650.016.6348865
GTCTAGG51750.016.275311
GTATAGG58850.016.0656571
GTACCGT30450.015.9958866
AGTACCG39250.015.8800135
TATACTG73900.015.8630795
TAGAGCG36750.015.8370875
GTGTAGG52650.015.6834331
GTGTATG75900.015.5572831
CGTCTTC728700.015.2486894-95
GTCTATG52550.015.006181
Produced by FastQC (version 0.11.5)