FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_FLW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_FLW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19667829
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT652150.027.09088594-95
TCTGCTT485350.025.77898494-95
CTTCTGC881250.025.02725294-95
TCTTCTG1120050.021.69312194-95
CTGCTTG315650.021.00446794-95
GTCTTCT1321000.019.0012894-95
CTAGACA58900.017.9922964
TAGACGG21550.017.6038275
CCTAGAC46000.017.211243
TATACCG45650.016.8914665
TAGAGCG41050.016.7752845
CGTCTTC1567950.016.30863494-95
TACACCG38650.015.7898785
GGACTGC38050.015.7137516
TGCTTGA236650.015.57753494-95
GTACCGT32050.015.3103646
GTCTAGG43550.015.1527781
GTATAAG68450.014.7622671
CCGTCTT1788700.014.75118194-95
TAGACCG28800.014.1745495
Produced by FastQC (version 0.11.5)