FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_FHUW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_FHUW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27700734
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGC628950.022.83172694-95
TTCTGCT475650.022.76257194-95
TCTTCTG790300.020.74623194-95
TCTGCTT382600.019.12897194-95
GTCTTCT928700.018.90582894-95
CCTAGAC77000.018.4436763
TAGACGG34050.017.9440165
TATACCG83400.016.4836445
CGTCTTC1082750.016.24895994-95
CTAGACA105750.015.9669774
GTGTATG115800.015.0836511
CCGTCTT1215900.014.86288392-93
GTCTAGG78350.014.756851
TAGAGCG71650.014.6349955
CTGCTTG270150.014.5728294-95
GTACCGT53200.014.3560566
CATAGAC100550.014.2471233
TACACAG72050.014.2099575
TACACCG61150.014.1774435
GTATAAG102200.014.1413791
Produced by FastQC (version 0.11.5)