FastQCFastQC Report
Wed 20 Nov 2019
H3CG5BCX3_l02_n01_FFS-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3CG5BCX3_l02_n01_FFS-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24879086
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGC488500.021.7682594-95
TTCTGCT375150.020.98441194-95
TAGACGG29150.020.390885
TCTTCTG592650.019.5979794-95
GTCTTCT686000.018.10069894-95
GTACCGT46500.017.9312466
TCTGCTT301050.017.57902394-95
TATACCG76700.017.0598775
CCTAGAC70150.016.4169353
CGTCTTC795150.016.38662794-95
GTCTAGG70800.015.9786881
AGTACCG61650.015.9351425
TAGAGCG62100.015.886145
CTAGACA96600.015.3829964
CCGTCTT893050.015.16869594-95
TACCGTG57500.015.0740947
TACACCG53300.014.714265
GCCGTCT1032350.014.35691394-95
GTATAAG99400.014.3303411
GACGGTC38700.014.2913617
Produced by FastQC (version 0.11.5)